Benchmarks
Retrospective dry-lab design quality metrics for Kairos v0.4.0. Every number is reproducible from the deterministic design tools — no opaque scoring.
Host fidelity
Kairos ranks CDS candidates for each target by how closely their codon usage resembles the host organism (Pichia). Within-protein ranking correlation (Spearman ρ) measures whether higher-scored candidates are consistently preferred.
≈ +0.35
Spearman correlation between Kairos codon reward and host-similarity, within-protein. Positive = higher-scored candidates are more host-like.
LOGO CV
Leave-one-group-out cross-validation. Retrospective — computed on existing data, not prospective validation.
Expression-construct optimization
Kairos learns element effects from expression-construct data. The learned effects are consistent with known biology: pCS1 and ERO1/SBH1 promoter/terminator elements show the expected directional impact. Cross-source generalization is measured across independent data cohorts.
≈ 0.12–0.26
Spearman ρ across cross-source folds. Range reflects method-dependent variation (within-source standardization on/off). Lower bound = conservative.
~627 records
21 cross-source folds. Retrospective calibrator evaluation; not a prospective predictor of expression or titer.
Coverage
Every design candidate is scored across multiple axes — each a deterministic, re-runnable computation:
Synthesis safety
Restriction sites, repeats, GC content, stop codons — hard constraints that block synthesis.
5′ translation initiation
Kozak context, secondary structure, start-codon accessibility.
Host codon fidelity
Codon adaptation to Pichia usage tables; within-protein ranking ρ ≈ +0.35.
Developability
Protein-level flags: aggregation tendency, glycosylation sites, stability signals.
Ask Kairos a research question — the dossier shows its evidence, scores, and provenance.