Evolrix AI — AI for synthetic biology manufacturing

Method

From question to design — one dry-lab loop

Kairos runs the design–build–test–learn cycle as a dry-lab loop today. Each stage declares what it is — retrieved evidence, model reasoning, or a deterministic tool.

01AskNatural-language research question› Natural-language question — e.g. express cytochrome c in Pichia, secreted?Live

02RetrieveCited evidence, with provenance› 66,000+ full-text papers · provenance on every cardLive

03DesignDeterministic CDS & leader panels› Deterministic CDS & leader panels — correct by constructionLive

04RankHost-fidelity scores you can re-run› Host-fidelity ρ ≈ +0.35 · scores you can re-runLive

05ReviewEvery claim audited — PASS / WARN / FAIL› 4 claims · 12 sources · verdict PASS (real dossier)Live

06FermentWet-lab validation closes the loop› Wet-lab validation via partner networkIn partnership

Product

One question in — a dossier you can audit

A Kairos session ends as a workbench dossier: cited evidence, a ranked design panel, structure checks, and a claim-by-claim audit. The demo below replays the published cytochrome-c run — every number real.

kairos workbench · live dossier

› Design a secreted expression construct for cytochrome c in Pichia

Research plan

Cytochrome c is a high-value heme protein — soluble recombinant production enables functional studies without mitochondrial isolation.

Signal peptide compatibility

Holo-cytochrome c maturation

Folding & strain engineering

AOX1 cassette optimization

About 1–2 minutesGo ahead →

Secretory signal peptide compatibility🚫 unproven

Holo-cytochrome c maturation in secretion🚫 unproven

Folding & strain engineering for secretion🚫 unproven

AOX1-driven expression cassette optimization🚫 unproven

Evidence too thin to call — Kairos says so instead of guessing.

Chat summary

Finding — secretion leader + host-weighted CDS ready; 12 synonymous candidates, host_weighted wins.

What doesn't help — evidence is thin on signal-peptide compatibility; all four hypotheses stay unproven.

94 evidence · 12 candidates · verdict PASS · 2 WARN

Open in workspace →

Evidence Design Structure Manufacture Audit

94 evidence · 6 retrieval rounds · EuropePMC 31 + KG 50 kept

Synergistic optimisation of expression, folding & secretion

PMID:33494776 · AppA phytase production in P. pastoris

Heterologous protein production in methylotrophic yeast

PMID:38730212 · recent progress review

Recombinant cytokines in K. phaffii

PMID:42113077 · design, production & purification

12 synonymous candidates · seed 42

#1host_weightedwinnerCopy CDS

#2gc_balancedtop tierCopy CDS

#3gc_balancedtop tierCopy CDS

Folding confidence screen

AlphaFold DB · 83 aa · mean pLDDT 91.2 — high confidence

lowpLDDThigh

Secreted construct · fed-batch AOX1 methanol induction

Centrifugation / microfiltration0.90

UF-DF (ultrafiltration / diafiltration)0.90

Spray drying0.85

Total recovery (multiplicative)0.689

Directional COGS $5–15/kg vs benchmark $5–50/kg · within parity range

Directional dry-lab estimates — absolute titer & recovery stay wet-lab.

verdict: PASS

7 claims · 94 sources — 5 PASS · 2 WARN

84 domain sources retrieved & citedevidence · EuropePMC/KGPASS

Candidate panel matches design outputdesign · deterministicPASS

Conversation-context statementreasoning · no sourceWARN

Signal-leader claim (P01149 / SUC2)target mismatchWARN

Generated Files · sources: EuropePMC ×31 · KG ×50 · every one linked

Walk through a real dossier, panel by panel

Evidence

Numbers we publish — and re-run

Dry-lab metrics we publish and re-run. Full methodology on /benchmarks/.

ρ ≈ +0.35

Within-protein host fidelity vs real titers (Spearman · 28 PichiaCLM variants, 5 proteins)

Minutes

From research question to ranked design panel

100%

Claims cited — then audited PASS / WARN / FAIL

Full metrics and methodology → /benchmarks/

Methods & benchmarks

Capabilities

What Kairos does

✓Ranks synthesis safety & host resemblance for Pichia constructs
✓Optimizes expression-construct elements — learned effects matching known biology (pCS1, ERO1/SBH1)
✓Scores 5′ translation initiation & protein developability
✓Checks structures in silico — AlphaFold DB + Boltz-2 confidence
✓Cites every claim to literature you can open and check
✓Audits every claim — automated PASS / WARN / FAIL verdict, line by line
✓Scores downstream manufacturability — fermentation, DSP recovery & directional cost, every number cited

Pichia-optimized dry-lab design, end to end — from cited evidence to an audited, order-ready construct. Bench validation runs through our partner network. Full scope statement →

Questions

What teams ask

FOR SCIENTISTSAudit the methodStructure-verification demo, benchmarks & the full scope statement. FOR INVESTORSRead the thesisThe thermodynamic-limit thesis, whitepaper, and published case studies. FOR BUILDERSTry it yourselfAsk Kairos a real research question — free self-serve access.

What is Evolrix AI?

An AI company building the tools that make synthetic biology computable — dramatically accelerating the path from research question to manufacturing-ready design. Designs that are provably correct, scores you can re-run, and answers grounded in literature. Built for teams that need provenance, not persuasion.

Who is it for?

Industrial R&D teams in synthetic biology, metabolic engineering, and biotech — from early-stage design to pre-manufacturing validation.

What can it do today?

Kairos v0.19.6 (Pichia-scoped): conversational research with cited evidence retrieval, LLM-backed reasoning, ranked CDS design panels with host-fidelity scores and expression-construct optimization (element effects matching known biology (pCS1, ERO1/SBH1)), secretion leader design, a downstream manufacturability panel (fermentation, DSP sequence, step-wise recovery, directional cost — every number cited), hypothesis-driven verification where every hypothesis receives a supported / contested / unverified / insufficient verdict, and an automated review layer that audits every claim with a PASS / WARN / FAIL verdict and also assesses whether each cited source actually supports the claim (same organism, same target, entailed or contradicted). Expanding to more hosts.

How does the chat work?

Ask a research question in natural language. Small talk gets an instant answer; quick questions get a fast cited reply; research questions go straight into the full pipeline. Kairos retrieves cited evidence, reasons with an LLM, runs deterministic design tools, and streams the result into a multi-panel workbench — evidence, design, structure, manufacturability, audit — with a 1–2 screen summary right in the chat and a deduplicated provenance manifest (PMID / DOI / patent / knowledge graph) under Generated Files. Each section carries its provenance, and the audit panel shows a claim-by-claim PASS / WARN / FAIL verdict.

What is Kairos scoped to?

Kairos ranks dry-lab design quality — synthesis safety, host resemblance, expression-construct optimization, 5′ translation initiation, and protein developability. Expression and titer are wet-lab questions, answered by wet-lab data.

Bring a target protein. Leave with a ranked design panel

Minutes, not weeks. Every construct scored, every claim cited.

Try Kairos Request a scoped pilot

Seeking the thermodynamic limit of biomanufacturing